The objective of the work was to establish a new, rapid and sensitive RP-HPLC method for determination of two commonly prescribed drug; miconazole and ornidazole. The RP-HPLC separation of the analytes was performed on a hypersil BDS C18 (250 mm x 4.6 mm, 5 µm) column, using isocratic elution of the mobile phase composed of potassium dihydrogen phosphate buffer (pH 3.5) and methanol (70:30 % v/v) with a flow rate of 1.0 ml/min. The multiple wavelength UV detector was set at a 230 nm for measurement of all compound. Quantification based on measuring the peak areas. The Two compound were resolved with retention times 4.227 for ornidazole and 5.413 for miconazole respectively. The calibration curves were linear in the range of 25-75 µg/ml and 5-15 µg/ml for ornidazole and miconazole respectively, all of them with coefficient of determination above 0.999. The methodology recoveries were higher than 99%. The limits of detection (LODs) were 1.140 µg/ml and 0.368 µg/ml for ornidazole and miconazole respectively. The intra-day coefficient of variation % RSD=0.213-0.667 and 0.254-1.40 for ornidazole and miconazole and inter-day coefficient of variation % RSD=0.717-1.408 and 1.24-1.74 for ornidazole and miconazole. The method was accurate, sensitive and simple for quality control as well as for stability indicating purpose.
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